MicroRNA expression profiling in the lungs of genetically different Ri chicken lines against the highly pathogenic avian influenza H5N1 virus

The highly pathogenic avian influenza (HPAI) virus triggers infectious diseases, resulting in pulmonary damage and high mortality domestic poultry worldwide. This study aimed to analyze miRNA expression profiles after infection with the HPAI H5N1 resistant susceptible lines of Ri chickens.For this purpose, Vietnamese chicken were used based on A/G allele <italic>Mx</italic> <italic>BF2</italic> genes. These genes are responsible for innate antiviral activity selected determine differentially expressed (DE) miRNAs HPAI-infected using small RNA sequencing. A total 44 3 days virus. Computational program analysis indicated candidate target possess significant functions related cytokines, chemokines, MAPK signaling pathway, ErBb Wnt pathway. Several DE miRNA-mRNA matches suggested play crucial roles mediating immune against viral evasion. results revealed potential regulatory response two lungs.